SELECTION AND RENAME MICROORGANISMS WITH HIGH CAPACITY OF PRODUCING AXIT LACTIC, ENZYME CELLULASE, XYLANASE, AMYLASE APPLYING FOR Canna edulis Ker RESIDUE AS ANIMAL FEEDS

Abstract

Canna edulis Ker. residue is a byproduct of its starch processing, accounting for 80% of fresh material product. Exploiting this material for fodder is an effective and realistic solution for not only the shortage of dry green fodder, but also increasing economic efficacy and decreasing environmental pollution in the C. edulis Ker. residue processing areas. The aim of this research is to choose microorganisms, which contains high acid lactic, in vivo enzymes (cellulase, xylanase, amylase) and inhibits pathogens to apply for C. edulis Ker. residue processing for fodder. As a result, there are 3 out of 20 experimental microorganisms named L. DM6; L. DR9 and L. DR27, that can produce high acid lactic, which amount is 14.3, 14.8, 15.4 mg/mL, in vivo enzymes and antibacterial at the high rate. Active amylase is expressed as substrate degrading zone diameter achieved at 18, 20, 25 mm, xylanase at 23, 20, 35 mm, cellulose at 20, 22, 30 mm. Antibacterial activity towards E. coli via substrate degrading zone diameter is 13, 16, 21 mm, to B. cereus is 7, 11 and 13 mm and to S. typhi is 12, 10 and 17 mm. Thanks to 16S rDNA sequencing methods for identification,  L. DM6 is found as Lactiplantbacillus plantarum, L. DR9 is Lactobacillus acidophilus and L. DR27 is Lactiplantbacillus plantarum.