ISOLATION, SELECTION, INDENTIFICATION OF SOME BENEFICIAL MICROORGANISMS AND INITIALLY APPLICED IN TREATMENT OF THE TOPS AND THE LEAVES SUGARCANE RESIDUES TO MAKE ORGANIC FERTILIZER

Abstract

This study aimed to isolate, select and identify beneficial microorganisms and initially apply them to treatment of by – product form the tops and the leafves of sugarcane to make organic fertilizer. The result of the study have been selected four bacterial species, including: I5, DK18.1, DK17.2, CAT1.2 which had strong cellulose-degrading activity and stability. These four microorganisms were isolated from naturally decomposed sugarcane leaf and soil samples. The method used to identify species was 16s rRNA gene sequencing method has identified I5, DK18.1, DK17.2, CAT1.2 as belonging to Bacillus amyloliquefaciens species (99.70% similarity); Bacillus velezensis (99.86% similarity); Bacillus amyloliquefaciens (99.93% similarity) and Bacillus siamensis (99.86% similarity). The results of using these microorganisms to treat the by – product form the leafves of sugarcane showed that the efficiency of decomposing organic substance after 38 days of decomposition was significantly improved; decomposed organic fertilizer products, mostly decomposed and rich in nutrients. The total nitrogen and effective phosphorus content in the finished product of the experimental formula using selected bacterial species was 1.37% and 0.77%, respectively, higher than the control formula (comparison formula) 0.92% and 0.49% respectively.