STUDY ON PROPAGATING PHU CUONG SWEET POTATO BY CELL TISSUE CULTURE METHOD

Abstract

Pho Cuong sweet potato is a type of sweet potato indigenous to Quang Ngai province that is degenerating due to asexual propagation over many generations. The goal of the research is to rapidly multiply in vitro to revive this potato variety. The growth peak of Pho Cuong sweet potato is used as a material to culture and create clean in vitro samples. Research results show that disinfection with Javen 33% (5 minutes) combined with treatment with HgCl₂ 0.1% (3 minutes) gives the highest disinfection efficiency. The suitable shoot regeneration environment is MS medium + 20 g/l sugar + 7 g/l agar + 2 mg/l NAA + 1 mg/l GA₃ + 1.5 mg/l BAP, for good shoot quality, average bud height reaches 5.71 cm with an average of 5.4 leaves/bud. The medium for rapid shoot multiplication when the sample is regenerated from the growth tip is MS medium + 20 g/l sugar + 7 g/l agar + 2 mg/l BAP + 1.5 mg/l kinetin + 2 mg/l GA₃ for. The best bud quality, with a bud multiplication factor of 3.33 times and an average bud height of 6.95 cm. The complete seedling creation environment for Pho Cuong sweet potato variety is MS medium + 20 g/l sugar + 7 g/l agar + 1 mg/l GA₃ + 1 mg/l NAA + 0.5 mg/l IAA suitable for the growth and development of seedlings, with an average of 3.15 roots/bud, an average root length of 10.24 cm and an average plant height of 7.91 cm.