Research to perfect in vitro culture technology for large - scale production of triploid Acacia hybrid clones (X101, D102)

Abstract

Studying on in vitro culture technology for the triploid Acacia X101 and X102 clones at the production scale was conducted. The research results showed that: During the clean explant stage, the use of excess or axillary buds sterilized with 5.0% NaClO in 8 minutes resulted in an effective budding rate of around 20% for both clones X101 and X102 was. The highest shoot/cluster was achieved in the improved Murashige and Skoog (MS*) medium + 1.0 mg/l BAP + 30 mg/l Ads (clone X101 had 5.54 shoots/cluster, clone X102 had 5.07 shoots/cluster). The highest effective shoot rate was achieved in MS* + 1.0 mg/l BAP + 0.25 mg/l GA3 + 0.05 mg/l NAA (clone X101 had 6.65 shoots/cluster, effective shoot rate: 87.78%, shoot height: 4.52 cm; X102 had 5.56 shoots/cluster, effective shoot rate: 87.78%, shoot height: 4.65 cm). The effective shoots qualified for root induction were in the ½ MS* medium + 2 mg/l IBA + 0.25 mg/l ABT with the rooting rate of 98% for both X101 and X102. After a 10 - days acclimatization period in the nursery, the survival rate of seedlings was 83.3% for X101 and 81.1% for X102. On the substrate consisting of 42% rice husk + 43% coconut fiber + 15% peat (self - digesting gourd shell), the survival rate was around 95%. Seedlings grew and developed well with NPK (13: 13: 13 TE) fertilizer at 1.0% concentration, diluted in 100 liters for 20,000 plants each 15 days.