CONSTRUCTION OF E. coli STRAINS WITH THE RGP GENE ENCODING THE SEA CUCUMBER GONAD-STIMULATING HORMONE

Abstract

Relaxin-like Gonad-stimulating Peptide (RGP) hormone is an advanced and effective solution in sea cucumber artificial breeding. In order to produce a large amount of RGP hormone with high biological activity, it is necessary to use recombinant protein technology through E. coli bacteria and P. pastoris yeast. Therefore, in this study, the pPIC9K vector was designed to carry the RGP gene (pPIC9K-RGP). The E. coli JM109 strain was constructed to carry the recombinant pPIC9K-RGP plasmid. After screening with the antibiotic ampicillin, E. coli colonies carrying the recombinant pPIC9K-RGP plasmid were produced. The DNA sequencing results of the pPIC9K-RGP plasmid from E. coli showed that the expression of the RGP gene encoding the sea cucumber reproductive stimulating hormone had been constructed in E. coli. This is an important step towards continuing the expression of the RGP hormone in P. pastoris yeast and the production of recombinant RGP hormone for sea cucumber breeding in Vietnam.