Screening and identifying mold strains capable of biosynthesizing enzyme cellulase and xylanase

Abstract

The aim of this study was to select mold strains capable of degrading cellulose and hemicellulose. The results showed that among 36 Trichoderma strains, 3 Trichoderma strains were selected with the highest ability to degrade cellulose and hemicellulose: Trichoderma D5 had cellulose and xylan substrate degrading zone diameters of 3.02 cm and 3.43 cm; Trichoderma D19 got 2.65 cm and 2.88 cm; Trichoderma D25 got 2.86 cm and 2.61 cm, respectively. Three Trichoderma strains had strong antagonistic activity against pathogenic fungi of Colletotrichum musae, Lasiodiplodia theobromae and Thielaviopsis paradoxa from 74.1 - 91.3%, 58.2 - 70.2% and 55.1 - 77.2%, respectively under in vitro conditions. The three strains were identified as Trichoderma viride (T. viride D5), Trichoderma harzianum (T. harzianum D19) and Trichoderma asperellum (T. asperellum D25) and were registered on GenBank with the corresponding code OR686312; OR690335 and OR686311. These strains were considered to have the potential to decompose organic materials with high cellulose and hemicellulose content such as C. edulis Ker. residue.