Isolation and evaluation of genetic diversity of some endophytic bacteria isolated from potato using RAPD and ISSR markers

Abstract

This study successfully isolated and investigated the effects of inoculation of 35 endophytic bacteria isolated from potato roots on the germination of Brassica integrifolia, from which 14 isolates were selected for genetic diversity analysis with 15 RAPD and 13 ISSR markers. The two groups of markers have amplified a total of 323 loci, in which the average rate of polymorphic loci reached 97.98% for the RAPD marker and 98.56% for the ISSR marker. 28 markers amplified 1.969 PCR products. The 14 isolated isolates had genetic diversity ranging from 0.34 - 0.96. At the genetic similarity coefficient of 0.57, the 14 microbial varieties were grouped into 3 main groups. It is concluded that the genetic diversity of the 14 isolated isolates is very high. The evaluated isolates can be used to study the effects of the inoculation on the performance of potato plants under in vitro conditions as well as in greenhouses or in the field.