Study on factors affecting the process of hydrolysis by enzyme pullulanase to increase the content of resistant starch from rice

Abstract

The study aimed to determine the optimal conditions to increase the content of digestive resistant starch by biological methods applying pullulanase enzyme to hydrolyze OM18 rice starch. The experiment was conducted to investigate the effects of factors affecting pullulanase enzyme activity: substrate concentration (10 - 50%), enzyme activity (10 - 50 U/g), hydrolysis time (2 - 10 hours), pH (4.0 - 6.0) and temperature (45 - 65^oC) to resistant starch content. The results determined the optimal conditions for pullulanase enzyme activity: substrate concentration (20%), enzyme activity (30 U/g), time hydrolysis (6 hours), pH (4.5), temperature (55^oC). The research results showed that the digestive resistant starch content of OM18 rice starch reached 12.2%. Research data is the premise for building a process to produce rice starch rich in digestive resistant starch content to apply to the processing of prebiotics food supplement products.