RESEARCH ON PROPAGATION OF Asarum glabrum Merr. BY IN VITRO

Abstract

Asarum glabrum Merr. (Aristolochiaceae) is an herbaceous plant used as a cough medicine, sore throat medicine and tonic, etc. They are being overexploited for their needs in domestic demand and selling across borders. Besides, deforestation also destroys their habitat. Asarum glabrum is ranked as Vulnerable (VU A1c,d) in the Vietnam Red Data Book (2007). Therefore, it is extremely necessary to apply in vitro propagation method - an advanced propagation method for Asarum glabrum. The results show that Asarum glabrum’s segments were sterilized with 0.1% HgCl₂ for 7 minutes, resulting in a clean sample rate of 81.11% and a clean sample budding rate of 77.78%. with 28 days. Its shoots were rapidly multiplied on Murashige & Skoog (MS) medium supplemented with 0.9 mg/l thidiazuron (TDZ), 30 g/l sucrose, 7 g/l agar for 100% of samples to create multiple shoots with the average shoot multiplication coefficient of 9.13 times and the average shoot height is 3.9 cm. Adding α-NAA at a concentration of 1.75 mg/l and IBA at a concentration of 0.5 mg/l to MS medium resulted in 86.67% of shoots taking root, with an average number of 2.77 roots and the average length of 0.65 cm. Initial research results show the possibility of applying in vitro methods to propagating the Asarum glabrum, creating a large number of high-quality sapdlings to supply the needs of growing and developing precious medicinal plants.