THE EFFECT OF FETAL BOVINE SERUM (FBS) CONCENTRATION ON CANINE STROMAL VASCULAR FRACTION CELL PROLIFERATION RATE

Abstract

This study examines the impact of fetal bovine serum (FBS) concentration on the proliferation of stromal vascular fraction (SVF) cells, a heterogeneous cell population enriched with mesenchymal stem cells (MSCs) and isolated from canine adipose tissue through 0.1% collagenase digestion. SVF cells were then cultured for 48 hours in media supplemented with FBS at concentrations of 0%, 3%, 5%, 8%, 10%. Optimal cell viability and proliferation were achieved with 8% FBS, resulting in the highest cell density of 51.17 x 10⁴ cells/mL (p < 0.05), with consistent cell morphology observed across all FBS conditions. These findings provide critical data for optimizing SVF culture conditions and validate the efficiency of collagenase digestion for MSC isolation from canine adipose tissue. The study underscores the potential of SVF and MSCs for clinical applications in canine disease management, particularly in regenerative veterinary medicine, with future research directed toward exploring SVF differentiation potential and broader therapeutic applications.