A STUDY ON THE EFFECTS OF SOME TISSUE - CULTURE MEDIUM ON THE ABILITY TO FORM SHOOTS AND ROOTS ON THE RED-FLESHED DRAGON FRUIT VARIETIES (Hylocereus costaricensis)

Abstract

The study aimed to find out the suitable tissue-culture medium, capable of creating shoots and roots from dragon fruit branch samples. Seven tissue-culture media have been tested for the purpose of bud production including: MS + BA (0.5 mg/L), MS + BA (0.5 mg/L) + NAA (0.1 mg/L), MS + BA (0.8 mg/L), MS + BA (0.8 mg/L) + NAA (0.1 mg/L), MS + BA (0.2 mg/L), MS + BA (0.2 mg/L) + NAA (0.1 mg/L) and MS (as a control); the results showed that the MS + BA (0.5 mg/L) treatment was the best with 9.67 shoots produced after 14 days of culture. Seven tissue-culture media have been tested for the purpose of rooting production including: MS 1/2 + NAA (0.5 mg/L), MS 1/2 + NAA (0.5 mg/L) + IBA (0.5 mg/L), MS 1/2 + NAA (0.8 mg/L), MS 1/2 + NAA (0.8 mg/L) + IBA (0.5 mg/L), MS 1/2 + NAA (0.2 mg/L), MS 1/2 + NAA (0.2 mg/L) + IBA (0.5 mg/L) and MS 1/2 (as a control); the results showed that the MS 1/2 + NAA (0.5 mg/L) + IBA (0.5 mg/L) treatment was the best with 9.36 roots produced after 28 days of culture.