Research on in vitro propagation of black ginger (Kaempferia parviflora Wall. Ex Baker)

Abstract

This study aims to determine the initial material and the effect of growth regulators on the micropropagation process of black ginger (Kaempferia parviflora) using tissue culture technique. The results showed that the suitable initial material was obtained from the buds of mature black ginger rhizomes or newly formed shoots from stem of the black ginger plant, which were sterilized with a 0.1% HgCl₂ solution for 15 minutes. The MS medium supplemented with 100 mg/L inositol, 30 g/L sucrose, 5.2 g/L agar, pH 5.6 - 5.8 and 3.0 mg/L BA was optimal for shoot multiplication, producing robust shoots with a multiplication rate of 4.5 and a height of 5.82 cm after 6 weeks of culture. Acclimatization involved placing culture bottles in a corridor for 3 days, then opening the lids and transferring to a net house for another 3 days before washing and planting, which resulted in a high survival rate and rapid root emergence within 8 days. A substrate mixture of soil, coconut coir and rice husk charcoal (2: 1: 1) was suitable for planting in vitro plantlets in the nursery, achieving a survival rate of 95.78% and an average plant height of 13.35 cm after 3 weeks.