IN VITRO MICROPROPAGATION OF OCHNA VARIETY “MAI VANG HUE” (Ochna integerrima (Lour.) Merr.)

Abstract

Ochna variety “Mai vang Hue” (Ochna integerrima (Lour.) Merr.) is currently an ornamental plant of great interest, so propagation for developing this variety is a necessary requirement. In vitro micropropagation method will produce a large number of plantlets that are uniform in genetics and age. With the materials being stem segments of ochna variety “Mai vang Hue”, the rate of shoot generation reached 66.7%, the shoot generation time was 24.3 days, the average number of shoots produced was 3.1 shoots/explant. Sterilizing the explants with NADCC1% for 15 minutes and NaDCC 0.5% for 5 minutes gave the highest regeneration rate of 66.7%, and the low infection rate of 13.3%. Medium ½ MS+ 30 g/l sucrose + 6.8 g/l agar + 2 mg/l Activated charcoal + 1 mg/l IBA is suitable for in vitro rooting of ochna variety “Mai vang Hue”, with a rooting rate of 100%, rooting time of 37 days, number of roots/explant is 5.1 roots and the plantlet quality is good.